Seahorse XF Cell Mito Stress Test Kit

The Agilent Seahorse Cell Mito Stress Test kit is the widely recognized, well accepted standard assay for assessing mitochondrial function. Multiple parameters are obtained in this one assay including, basal respiration, ATP-linked respiration, maximal and reserve capacities, and non-mitochondrial respiration. The assay provides insights into the mechanism of mitochondrial dysfunction and allow users to investigate functional differences among cell types, drug candidates, and genetic or biochemical interventions.

Each kit contains the material sufficient for six full plate tests and is available in two packaging sizes. The XF kit is for use with the Agilent Seahorse XF Pro, XF96/XFe96 and XFe24 Analyzers. The XFp kit is for use with the Agilent Seahorse XF HS Mini and XFp Analyzers.

• XF Assay Kits, Reagents & Cell Assay Media

Product Details

• The assay measures basal respiration, ATP-linked respiration, H + (proton) leak, maximal respiration, spare respiratory capacity, and non-mitochondrial respiration using modulators of cellular respiration
• Compounds are reconstituted directly in the assay medium, eliminating the need for DMSO or other solvents
• Each lot is rigorously tested and validated with functional assays to deliver consistent and robust results, eliminating the costs associated with failed experiments
• Upload your assay result file to your Agilent Seahorse Analytics account to calculate and report parameters of the XF Cell Mito Stress Test, share with collaborators, or export data to Microsoft Excel or GraphPad Prism for other graphing or analysis needs

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Automatic measurement of energy metabolism in real time

Agilent Seahorse XF Analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis. Seahorse XF Analyzers perform compound addition and mixing, label-free analytical detection, and automatic measurement of OCR and ECAR in real time.

Gaining Insights into Disease Biology for Target Identification and Validation

This Application Note shows how Seahorse XF technology can be used to determine specific metabolic states associated with cellular dysfunction and disease

• Application Notes
• English
• 12 Nov 2019
• 572.72 KB
• PDF

Direct Measurements of Cellular Metabolism for Identification of Mitochondrial Drug Targets

An overview of how the Agilent Seahorse XF Cell Mito Stress Test and XF PMP workflows can be applied to mitochondrial and metabolic target identification studies.

• Application Notes
• English
• 10 Jun 2019
• 389.41 KB
• PDF

Identifying Metabolic Switches Using an Agilent Seahorse XFe Analyzer in Hypoxia

Identifying Metabolic Phenotype Switches in Cancer Cells Using the Agilent Seahorse XF/XFe Analyzer in an Hypoxic Environment

• Application Notes
• English
• 03 Jan 2017
• 744.28 KB
• PDF

Measure Mitochondrial Respiration in Skeletal Muscle Fibers with a Seahorse Analyzer

Measuring Mitochondrial Respiration in Intact Skeletal Muscle Fibers with an Agilent Seahorse XF24/XFe24 Analyzer

• Application Notes
• English
• 22 Dec 2016
• 1.07 MB
• PDF

Assessing therapies for traumatic brain or spinal cord injuries

XF technology provides insight into treatment interventions for mitochondrial dysfunction

• Application Notes
• English
• 22 Dec 2016
• 679.93 KB
• PDF

Assessing the impact of autophagy on cellular metabolism

Real-time, functional metabolic assays connect energy pathways and autophagy

• Application Notes
• English
• 22 Dec 2016
• 1.06 MB
• PDF

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How are the metabolic parameters calculated in the Seahorse XF Report Generators?

Why is a 1-hour non-CO₂ incubation necessary for the cell plate?

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Agilent Seahorse XF Instruments Selection Guide

Evaluate and compare the Seahorse XF Analyzers.

Agilent Cell Analysis Publication Database

Search scientific publications by research area, cell type and more.

Seahorse XF Assay Learning Center

Up-to-date information on protocols and procedures for preparing, running, and analyzing an XF assay.

Customer Reviews for Seahorse XF Cell Mito Stress Test Kit

Explore customer reviews of this product or write your own review

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The Agilent Seahorse XF Pro platform

Transforming Bioenergetics - Again. Featuring new pharma-oriented workflow solutions with improved precision, along with advanced experimental design and analysis tools, you can optimize your workflow from start to finish.

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